Supercritical Fluid Fractionation for Enhancement of Insecticidal Activity of Eucalyptus Extract

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F13%3A00394562" target="_blank" >RIV/67985858:_____/13:00394562 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Supercritical Fluid Fractionation for Enhancement of Insecticidal Activity of Eucalyptus Extract
Original language description
The objective of this work was to optimize the pressure of supercritical CO2 extraction (40 °C, 9-30 MPa) of eucalyptus leaves to reach maximal insecticidal activity of extract. Several methods of extract fractionation were applied to increase the concentration of volatiles in extract. Firstly, a time fractionation with gradually increased solvent density was carried out using one separator. In addition, the SFE combined with extract fractionation in two separators and fractionation with use of appropriate sorbent was applied. The efficiency of SFE in terms of volatile fraction composition, yield and insecticidal activity was compared with hydrodistillation. The composition of volatiles in the isolates was determined by GC-MS and GC-FID. The insecticidal activity of isolates was measured on larvae of Spodoptera litoralis in terms of antifeedancy (ED50), acute and chronic toxicity (LD50). Yield, composition and insecticidal activity of extracts were strongly influenced by the experiment
Czech name
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Czech description
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Project
<a href="/en/project/TA01010578" target="_blank" >TA01010578: Research and development of new products for complex protection of plants based on natural substances obtained using supercritical fluid extraction and hydrodistillation</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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