Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F12%3A00396508" target="_blank" >RIV/67985882:_____/12:00396508 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00216-012-6308-9" target="_blank" >http://dx.doi.org/10.1007/s00216-012-6308-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-012-6308-9" target="_blank" >10.1007/s00216-012-6308-9</a>

Result language

angličtina

Original language name

Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma

Original language description

We report on the use of new biofunctionalized gold nanoparticles (bio-AuNPs) that enable a surface plasmon resonance (SPR) biosensor to detect low levels of carcinoembryonic antigen (CEA) in human blood plasma. Bio-AuNPs consist of gold nanoparticles functionalized both with (1) streptavidin, to provide high affinity for the biotinylated secondary antibody used in the second step of the CEA sandwich assay, and with (2) bovine serum albumin, to minimize the nonspecific interaction of the bio-AuNPs with complex samples (blood plasma). We demonstrate that this approach makes it possible for the SPR biosensor to detect CEA in blood plasma at concentrations as low as 0.1 ng/mL, well below normal physiological levels (approximately nanograms per milliliter).Moreover, the limit of detection achieved using this approach is better by a factor of more than 1,000 than limits of detection reported so far for CEA in blood plasma using SPR biosensors.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JB - Sensors, detecting elements, measurement and regulation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

10

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

2869-2875

UT code for WoS article

000311310100010

EID of the result in the Scopus database

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