Optical imaging and localization of prospective scattering labels smaller than a single protein

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F19%3A00504562" target="_blank" >RIV/67985882:_____/19:00504562 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0030399218310818?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0030399218310818?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.optlastec.2018.08.014" target="_blank" >10.1016/j.optlastec.2018.08.014</a>

Result language
angličtina
Original language name
Optical imaging and localization of prospective scattering labels smaller than a single protein
Original language description
Labeling of biological matter is a key technique enabling visualization and real-time tracking of dynamic processes beyond the diffraction limit. While the precision of fluorescence-based localization microscopy is limited by signal saturation, a new generation of small scattering labels (such as gold nanoparticles) have brought the localization precision below the size of a single protein. However, the size of scattering labels is also the most obvious handicap, as they are usually significantly larger than the resulting localization precision. We address this inconsistency and demonstrate detection and precise localization of gold nanoparticles of only 2 nm in diameter using interferometric detection of scattering (iSCAT). This size is considerably smaller than the size of a small protein and in combination with the possibility of tracking the nanoparticle position, we foresee a new concept of detailed studies of biomolecular systems in the future
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)

Project
<a href="/en/project/LL1602" target="_blank" >LL1602: Optical imaging of single protein dynamics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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