Interferometric scattering (iSCAT) microscopy with optimized reference wave
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F19%3A00518803" target="_blank" >RIV/67985882:_____/19:00518803 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1117/12.2522360" target="_blank" >http://dx.doi.org/10.1117/12.2522360</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2522360" target="_blank" >10.1117/12.2522360</a>
Alternative languages
Result language
angličtina
Original language name
Interferometric scattering (iSCAT) microscopy with optimized reference wave
Original language description
The understanding of nanoscale biological processes is limited by the level of details we can achieve when observing their dynamics. Addressing molecules of interest using fluorescent labels is the most common contrast mechanism in biological nano-imaging. However, the complex photophysics of fluorescent labels limits the localization precision as well as observation times in practical experiments. As an alternative to fluorescence-based microscopy interferometric scattering microscopy (iSCAT) was recently introduced. It is an optical microscopy technique allowing to detect and track nanoscale objects with sub-nanometre localization precision. The basic concept of this technique is the interference of light scattered on the particle with a reference wave light partially reflected at the microscopic slide. Recent advancements pushed the sensitivity and high-speed tracking down to a level of a single unlabelled protein by balancing the amplitudes of scattering and reference waves. This is often achieved by optimizing the reference wave, e.g. via placing a partially transparent mask near the back focal plane of a high numerical aperture microscope. In this contribution we introduce and demonstrate an innovative layout of the iSCAT microscope with optimized reference wave and minimized interferometric artefacts. We benchmark the detection capabilities of the new layout using series of extremely small spherical gold nanoparticles and demonstrate possible applications of the novel detection scheme
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/LL1602" target="_blank" >LL1602: Optical imaging of single protein dynamics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proc. of SPIE, Optical Sensors 2019
ISBN
978-1-5106-2723-9
ISSN
0277-786X
e-ISSN
1996-756X
Number of pages
5
Pages from-to
110282P
Publisher name
SPIE
Place of publication
Bellingham
Event location
Praha
Event date
Apr 1, 2019
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000484773500061