In vitro study of interaction of 17 beta-hydroxysteroid dehydrogenase type 10 and cyclophilin D and its potential implications for Alzheimer's disease

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F19%3A00518381" target="_blank" >RIV/67985882:_____/19:00518381 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-019-53157-7" target="_blank" >https://www.nature.com/articles/s41598-019-53157-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-019-53157-7" target="_blank" >10.1038/s41598-019-53157-7</a>

Result language
angličtina
Original language name
In vitro study of interaction of 17 beta-hydroxysteroid dehydrogenase type 10 and cyclophilin D and its potential implications for Alzheimer's disease
Original language description
In early stages of Alzheimer's disease (AD), amyloid-beta (A beta) accumulates in neuronal mitochondria where it interacts with a number of biomolecules including 17beta-hydroxysteroide dehydrogenase 10 (17 beta-HSD10) and cyclophilin D (cypD). It has been hypothesized that 17 beta-HSD10 interacts with cypD preventing it from opening mitochondrial permeability transition pores and that its regulation during AD may be affected by the accumulation of A beta. In this work, we demonstrate for the first time that 17 beta-HSD10 and cypD form a stable complex in vitro. Furthermore, we show that factors, such as pH, ionic environment and the presence of A beta, affect the ability of 17 beta-HSD10 to bind cypD. We demonstrate that K+ and Mg2+ ions present at low levels may facilitate this binding. We also show that different fragments of A beta (A beta(1-40) and A beta(1-42)) affect the interaction between 17 beta-HSD10 and cypD differently and that A beta(1-42) (in contrast to A beta(1-40)) is capable of simultaneously binding both 17 beta-HSD10 and cypD in a tricomplex.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/NV16-27611A" target="_blank" >NV16-27611A: Interactions of intracellular amyloid beta and diagnosis of Alzheimer disease</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)