Ionic Environment Affects Biomolecular Interactions of Amyloid-beta: SPR Biosensor Study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F20%3A00537414" target="_blank" >RIV/67985882:_____/20:00537414 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.3390/ijms21249727" target="_blank" >https://doi.org/10.3390/ijms21249727</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21249727" target="_blank" >10.3390/ijms21249727</a>

Result language

angličtina

Original language name

Ionic Environment Affects Biomolecular Interactions of Amyloid-beta: SPR Biosensor Study

Original language description

In early stages of Alzheimer's disease (AD), amyloid beta (A beta) accumulates in the mitochondrial matrix and interacts with mitochondrial proteins, such as cyclophilin D (cypD) and 17 beta-hydroxysteroid dehydrogenase 10 (17 beta-HSD10). Multiple processes associated with AD such as increased production or oligomerization of A beta affect these interactions and disbalance the equilibrium between the biomolecules, which contributes to mitochondrial dysfunction. Here, we investigate the effect of the ionic environment on the interactions of A beta (A beta(1-40), A beta(1-42)) with cypD and 17 beta-HSD10 using a surface plasmon resonance (SPR) biosensor. We show that changes in concentrations of K+ and Mg2+ significantly affect the interactions and may increase the binding efficiency between the biomolecules by up to 35% and 65% for the interactions with A beta(1-40) and A beta(1-42), respectively, in comparison with the physiological state. We also demonstrate that while the binding of A beta(1-40) to cypD and 17 beta-HSD10 takes place preferentially around the physiological concentrations of ions, decreased concentrations of K+ and increased concentrations of Mg2+ promote the interaction of both mitochondrial proteins with A beta(1-42). These results suggest that the ionic environment represents an important factor that should be considered in the investigation of biomolecular interactions taking place in the mitochondrial matrix under physiological as well as AD-associated conditions

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10306 - Optics (including laser optics and quantum optics)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Volume of the periodical

21

Issue of the periodical within the volume

24

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

9727

UT code for WoS article

000602991100001

EID of the result in the Scopus database

2-s2.0-85098152669