Hsp70 Trap Assay for Detection of Misfolded Subproteome Related to Myelodysplastic Syndromes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F19%3A00518388" target="_blank" >RIV/67985882:_____/19:00518388 - isvavai.cz</a>
Alternative codes found
RIV/00023736:_____/19:00012825
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.analchem.9b04175" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.analchem.9b04175</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.9b04175" target="_blank" >10.1021/acs.analchem.9b04175</a>
Alternative languages
Result language
angličtina
Original language name
Hsp70 Trap Assay for Detection of Misfolded Subproteome Related to Myelodysplastic Syndromes
Original language description
The onset and progression of numerous serious diseases (e.g., various types of malignancies, neuro-degenerative diseases, and cardiac diseases) are, on a molecular level, associated with protein modifications and misfolding. Current methods for the detection of misfolded proteins are not able to detect the whole misfolded subproteome and, moreover, are rather laborious and time consuming. Herein, we report on a novel simple method for the detection of misfolded proteins employing a surface plasmon resonance (SPR) biosensor and heat shock protein 70 (Hsp70) that recognizes and traps misfolded proteins in a nucleotide-dependent manner. We use this method for the detection of misfolded proteins in blood plasma of patients with various subtypes of myelodysplastic syndromes (MDS) and healthy donors. Our results reveal significantly elevated levels of misfolded proteins in the two stages of MDS that are most affected by oxidative stress: low-risk (RARS) and intermediate-risk (RCMD) patients. This approach can be extended to a variety of diseases and provides unique insights into the thus far unexplored area of blood proteome.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
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Volume of the periodical
91
Issue of the periodical within the volume
22
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
14226-14230
UT code for WoS article
000498280100012
EID of the result in the Scopus database
2-s2.0-85074876787