Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F20%3A00538002" target="_blank" >RIV/67985882:_____/20:00538002 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.jphotobiol.2020.111812" target="_blank" >https://doi.org/10.1016/j.jphotobiol.2020.111812</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jphotobiol.2020.111812" target="_blank" >10.1016/j.jphotobiol.2020.111812</a>

Result language

angličtina

Original language name

Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers

Original language description

One of the most important barriers to the detection of the biological autoluminescence (BAL) from biosystems using a non-invasive monitoring approach, in both the in vivo and the in vitro applications, is its very low signal intensity ( < 1000 photons/s/cm(2)). Experimental studies have revealed that the formation of electron excited species, as a result of reactions of biomolecules with reactive oxygen species (ROS), is the principal biochemical source of the BAL which occurs during the cell metabolism. Mitochondria, as the most important organelles involved in oxidative metabolism, are considered to be the main intracellular BAL source. Hence, in order to achieve the BAL enhancement via affecting the mitochondria, we prepared a novel mitochondrial-liposomal nanocarrier with two attractive features including the intra-liposomal gold nanoparticle synthesizing ability and the mitochondria penetration capability. The results indicate that these nanocarriers (with the average size of 131.1 +/- 20.1 nm) are not only able to synthesize the gold nanoparticles within them (with the average size of 15 nm) and penetrate into the U2OS cell mitochondria, but they are also able to amplify the BAL signals. Our results open new possibilities for the use of biological autoluminescence as a non-invasive and label-free monitoring method in nanomedicine and biotechnology

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20201 - Electrical and electronic engineering

Project

<a href="/en/project/GA18-23597S" target="_blank" >GA18-23597S: High-frequency microdevices for controlling protein nanomotors</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Photochemistry and Photobiology. B - Biology Section

ISSN

1011-1344

e-ISSN

—

Volume of the periodical

204

Issue of the periodical within the volume

March

Country of publishing house

CH - SWITZERLAND

Number of pages

7

Pages from-to

111812

UT code for WoS article

000518871200029

EID of the result in the Scopus database

2-s2.0-85079147459