Multiscale modeling and analysis for high-fidelity interferometric scattering microscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F21%3A00543290" target="_blank" >RIV/67985882:_____/21:00543290 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1088/1361-6463/abf70d" target="_blank" >https://doi.org/10.1088/1361-6463/abf70d</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1088/1361-6463/abf70d" target="_blank" >10.1088/1361-6463/abf70d</a>

Result language
angličtina
Original language name
Multiscale modeling and analysis for high-fidelity interferometric scattering microscopy
Original language description
Interferometric scattering microscopy (iSCAT), as an ultrasensitive fluorescence-free imaging modality, has recently gained enormous attention and has been rapidly developing from demonstration of principle to quantitative sensing and bioanalytics. Here we report on a theoretical framework of multiscale modeling and analysis for iSCAT with samples of arbitrary shapes under any types of illumination and detection schemes. We theoretically predict and experimentally confirm different evolution behaviors of the interference contrast as a function of the axial defocusing for dielectric and metallic nanoparticles. We provide a transparent understanding of the origin of the interference phenomenon in terms of plane wave components and explain how the interference contrast changes with the size and material of the nanoprobe and the numerical aperture of the microscope objective. Moreover, we investigate a sample system mimicking a gold nanoparticle in a simplified cell environment and show the position-dependent and asymmetric point spread function of the nanoparticle
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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