Tyrosine-Capped Pt Nanozyme Functionalized with cDNA: An Innovative Sensor Designed for One-Step Detection of the miRNA-21 Biomarker
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F25%3A00638970" target="_blank" >RIV/67985882:_____/25:00638970 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/25:43927410 RIV/00216305:26620/26:0198636
Result on the web
<a href="https://doi.org/10.1021/acsomega.5c05564" target="_blank" >https://doi.org/10.1021/acsomega.5c05564</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsomega.5c05564" target="_blank" >10.1021/acsomega.5c05564</a>
Alternative languages
Result language
angličtina
Original language name
Tyrosine-Capped Pt Nanozyme Functionalized with cDNA: An Innovative Sensor Designed for One-Step Detection of the miRNA-21 Biomarker
Original language description
The platinum nanozymes capped with tyrosine (Pt-Tyr NZ) were synthesized which demonstrated peroxidase and oxidase catalytic activities toward 3,3 ',5,5 '-tetramethylbenzidine (TMB). The kinetic analyses revealed that Pt-Tyr NZ exhibited higher catalytic activity and substrate affinity for both H2O2 and TMB compared to horseradish peroxidase (HRP), indicating its superior efficiency in biosensing applications. The synthesized Pt-Tyr NZ was reversibly conjugated with complementary DNA (cDNA), which can selectively hybridize with miRNA-21, a cancer biomarker, and after hybridization with miRNA-21, detaches from the surface of Pt-Tyr NZ. Upon cDNA conjugation, the catalytic activity of Pt-Tyr NZ decreased, but it was restored in the presence of miRNA-21, providing a one-step spectrophotometric biosensing method for miRNA-21 determination. Optimization of parameters affecting the sensitivity of the developed method for miRNA-21 detection was performed, including the tyrosine-to-platinum ratio in Pt-Tyr NZ synthesis, Pt-Tyr NZ concentration, and cDNA concentration for conjugation. The developed method was fully validated and showed promising results in terms of reproducibility (RSD = 1.26%, 95% confidence interval = 0.03, n = 6), repeatability (RSD = 0.83%, n = 5), and accuracy for spiked miRNA-21 determination in 10% filtered human serum samples (recovery rate = 109.7 +/- 4.2%). It showed a linear dynamic range (LDR) from 37.1 to 185 nM, with a limit of detection (LOD) of 11.1 nM and a limit of quantification (LOQ) of 36.9 nM
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Omega
ISSN
2470-1343
e-ISSN
2470-1343
Volume of the periodical
10
Issue of the periodical within the volume
35
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
40324-40334
UT code for WoS article
001555731500001
EID of the result in the Scopus database
2-s2.0-105015513190