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The Influence of Purification of Carp Collagen Used in a Novel Composite Graft with Sandwich Construction of the Wall on Its Biological Properties and Graft Patency Rates

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F19%3A00511739" target="_blank" >RIV/67985891:_____/19:00511739 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11110/19:10398593 RIV/68407700:21220/19:00336906 RIV/00023001:_____/19:00078319 RIV/00064165:_____/19:10398593

  • Result on the web

    <a href="https://www.biomed.cas.cz/physiolres/pdf/2019/68_603.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2019/68_603.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.33549/physiolres.934117" target="_blank" >10.33549/physiolres.934117</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The Influence of Purification of Carp Collagen Used in a Novel Composite Graft with Sandwich Construction of the Wall on Its Biological Properties and Graft Patency Rates

  • Original language description

    We compared graft outcome between two types of a novel composite three-layer carp-collagen-coated vascular graft in low-flow conditions in a sheep model. Collagen in group A underwent more cycles of purification than in group B in order to increase the ratio between collagen and residual fat. The grafts were implanted end-to-side in both carotid arteries in sheep (14 grafts in 7 sheep in group A, 18 grafts in 9 sheep in group B) and artificially stenosed on the right side. The flow in the grafts in group A decreased from 297 +/- 118 ml/min to 158 +/- 159 ml/min (p=0.041) after placement of the artificial stenosis in group A, and from 330 +/- 164ml/min to 97 +/- 29 ml/min (p=0.0052) in group B (p=0.27 between the groups). From the five surviving animals in group A, both grafts occluded in one animal 3 and 14 days after implantation. In group B, from the six surviving animals, only one graft on the left side remained patent (p=0.0017). Histology showed degradation of the intimal layer in the center with endothelization from the periphery in group A and formation of thick fibrous intimal layer in group B. We conclude that the ratio between collagen and lipid content in the novel three-layer graft plays a critical role in its patency and structural changes in vivo.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    20601 - Medical engineering

Result continuities

  • Project

    <a href="/en/project/NV15-27941A" target="_blank" >NV15-27941A: The use of nonatigen fish collagen in construction of implants and as drug carriers.</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Physiological Research

  • ISSN

    0862-8408

  • e-ISSN

  • Volume of the periodical

    68

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    8

  • Pages from-to

    603-610

  • UT code for WoS article

    000483374200008

  • EID of the result in the Scopus database

    2-s2.0-85071715123