The proportion of the key components analysed in collagen-based isolates from fish and mammalian tissues processed by different protocols

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F21%3A00545736" target="_blank" >RIV/67985891:_____/21:00545736 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21220/21:00357105
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0889157521002593?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0889157521002593?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jfca.2021.104059" target="_blank" >10.1016/j.jfca.2021.104059</a>

Result language
angličtina
Original language name
The proportion of the key components analysed in collagen-based isolates from fish and mammalian tissues processed by different protocols
Original language description
The aim of this pilot study is to compare two extraction processes tested in our lab with intention to preserve the biological structure and functions of collagen-based isolate for biomedical purposes during its processing. Collagen type I was isolated from various animal sources from fish, porcine and rat skins. The resulting isolates, along with commercial calf collagens as control, were subsequently characterised and their composition examined by means of several analytical methods such as amino acid analysis, FTIR, SDS-PAGE. Moreover, the moisture, lipid, glycosaminoglycans, hydroxyproline and N contents were determined. We found that both the factors considered, the source of the collagen and the process employed, exerted effects on the final composition of the isolate and we monitored the key differences, in particular, with respect to the protein and lipid contents. Moreover, the study provides a discussion of issues surrounding the assessment of the actual pure collagen content determined on the basis of the content of hydroxyproline and the purity of the protein fraction.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Project
<a href="/en/project/NV19-02-00068" target="_blank" >NV19-02-00068: Bioartificial cardiovascular patches and vessels from porcine collagen reinforced with nano/microfibers using stem cells and dynamic culture</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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