Plasmids of Selenomonas ruminantium and development of host-vector system

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F01%3A00347927" target="_blank" >RIV/67985904:_____/01:00347927 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/01:00348009
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Plasmids of Selenomonas ruminantium and development of host-vector system
Original language description
A high frequency of plasmids was detected in the rumen bacterium Selenomonas ruminantium. Plasmids 0.9-20 kb in size were detected in more than 50 % tested strains. Densitometric analysis indicated that plasmid DNA could represents more than 25 % of total cellular DNA. Up to six plasmids were detected in strain S. ruminantium 18. Two smallest cryptic plasmids pSRD181 and pSRD182 from this strain were cloned into Escherichia coh vector pBluescriptSK(+) and partially characterized. The plasmid pSRD181 is1.4 kb and pSRD182 is 2.0 kb. While computer analysis of pSRD181 sequence data showed high homology with replication protein of Staphylococcus aureus plasmids, the pSRD182 sequence showed no significant homology in GenBank data, Strain S. ruminantium 28was successfully transformed with pJW1 derived plasmid pJIB1 using ampicillin resistance gene as marker This is the first report on transformation of selenomonads with foreign DNA.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)