Protein fingerprints of anti-cancer effects of cyclin-dependent kinase inhibition: identification of candidate biomarkers using 2-D liquid phase separation coupled to mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F05%3A00022712" target="_blank" >RIV/67985904:_____/05:00022712 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/05:00001923
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Protein fingerprints of anti-cancer effects of cyclin-dependent kinase inhibition: identification of candidate biomarkers using 2-D liquid phase separation coupled to mass spectrometry
Original language description
The purpose of this study was to apply a recently introduced proteomic based approach to identify candidate biomarkers of the response to anticancer activity of cyclin-dependent kinase inhibitor, bohemine. Mapping of the total protein expression of CEM lymphoblastic leukemia cells following bohemine treatment was performed by 2-D liquid phase separation. 2-D protein expression maps of control untreated and bohemine treated cells were generated and inter-sample comparison was performed. Most of the differentially expressed proteins representing candidate biomarkers of cancer cell response to the treatment were selected for identification by mass spectrometry. Our results demonstrating down regulation of three histone variants, different in their pI andhydrophobicity, in response to bohemine indicated that anti-mitotic and anti-cancer activities of this compound may be associated with epigenetic regulation at the level of chromatin structure. Furthermore, crk-like adaptor scaffolding...
Czech name
Proteinové otisky protinádorových účinků inhibice cdk: identifikace potenciálních biomarkerů s využitím 2-D kapalinové separace a hmotnostní spektrometrie
Czech description
Účelem studie bylo využití nedávno vyvinutého proteomového postupu k identifikaci potenciálních biomarkerů charakteristických pro odpověd nádorových buněk na inhibitor cyklin-dependeních kináz, bohemin. Mapování celkových proteinových profilů bylo provedeno pomocí ProteomLb a PF 2D systému pracujícího na bázi 2.D kapalinové chromatografie.Většina proteinů ovlivněných boheminem identifikovány hmotnostní spektrometrií. Změny tří nrůzných forem histonů indikují, že antimitotické a protinádorové působení boheminu souvisí s epigenetickou regulací na úrovni chromatinu.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA301%2F05%2F0418" target="_blank" >GA301/05/0418: New generations of cyclin-dependent kinase inhibitors with significant anticancer properties</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Technology in Cancer Research and Treatment
ISSN
1533-0346
e-ISSN
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Volume of the periodical
4
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
447-454
UT code for WoS article
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EID of the result in the Scopus database
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