Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F06%3A00040840" target="_blank" >RIV/67985904:_____/06:00040840 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP

Original language description

The suitability of restriction fragment lenght polymorphism (RFLP) analysis of the ribosomal DNA cluster for discriminating two genera of anaerobic polycentric fungi, Orcinomyces and Anaeromyces, was determined. Three PCR-amplified DNA fragments - nuclear small subunit 18S and the nuclear large subunit S28 and internal transcribed spacer (ITS)-were restricted with endonucleases AluI, HinfI, DraI. Although the SSU DNA fragment could be restricted successfully by all four enzymes, no differences were observed between restriction patterns of Orpinomyces and Anaeromyces. Genus-specific RFLP patterns were determined for Orpinomyces and Anaeromyces genera; the results showed that the PCR-RFLP analysis of rDNA offers an easy and rapid tool for differentiationof two polycentric genera of anaerobic fungi, which could be hardly separated on the basis of morphology.

Czech name

Diferenciace anaerobních polycentrických hub na základe rDNA PCR-RFLP analýzy

Czech description

PCR produkty z malé 18S a velké 28S ribozomální podjednotky a ITS spaceru byly podrobeny restrikční analýze za použití restrikčních enzymů AluI, HinfI, DraI. Rody Orpinomyses a Anaeromyces se nelišily v RFLP profilu pro malou ribozomální podjednotku 18S,avšak vykazovaly rozdílné restrikční profily při štěpení fragmentů podjednotky 28S a ITS fragmentu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA523%2F05%2F2555" target="_blank" >GA523/05/2555: Deep phylogeny of anaerobic fungi</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

273-277

UT code for WoS article

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EID of the result in the Scopus database

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