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Detection of Possible AI-2-mediated Quorum Sensing System in Commensal Intestinal Bacteria

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F08%3A00309954" target="_blank" >RIV/67985904:_____/08:00309954 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Detection of Possible AI-2-mediated Quorum Sensing System in Commensal Intestinal Bacteria

  • Original language description

    The Vibrio harveyi BB170 autoinducer bioassay was used to detect possible quorum sensing autoinducer 2 molecule (AI-2) in culture fluids of commensal intestinal bacteria. The assay showed that the culture fluids of Bacteroides vulgatus, Clostridium proteoclasticum, Escherichia coli, Eubacterium rectale, Lachnospira multipara, Pseudobutyrivibrio ruminis, Roseburia intestinalis, Ruminococcus albus and Ruminococcus flavefaciens contained AI-2-like molecules. Furthermore, we could also amplify bands from some of the tested strains using primers designed to the luxS gene. These findings suggest that the AI-2 molecule is present in the gastrointestinal tract, but it was not proved up to now that is used for bacterial cell-to-cell communication.

  • Czech name

    Důkaz přítomnosti článků AI-2 quorum sensing systému u střevních bakterií lidí

  • Czech description

    U komensálních střevních bakterií byla pomocí autoindukčního systému Vibrio harveyi BB170 testována přítomnost molekul AI-2 systému pro quorum sensing. V kulturách Bacteroides vulgatus, Clostridium proteoclasticum, Escherichia coli, Eubacterium rectale,Lachnospira multipara, Pseudobutyrivibrio ruminis, Roseburia intestinalis, Ruminococcus albus a Ruminococcus flavefaciens byla prokázána přítomnost látek podobných AI-2. Navíc se podařilo pomocí primerů specifických pro luxS gen prokázat přítomnost tohoto genu ve většině testovaných kultur. To znamená, že součásti systému quorum sensing jsou u nich přítomny, pouze se zatím neprokázalo, že slouží ke komunikaci mezi buňkami.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA303%2F06%2F0974" target="_blank" >GA303/06/0974: Interaction of commensal and probiotic bacteria with gut mucosa studied in gnotobiotic models</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Folia Microbiologica

  • ISSN

    0015-5632

  • e-ISSN

  • Volume of the periodical

    53

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    4

  • Pages from-to

  • UT code for WoS article

    000257941200009

  • EID of the result in the Scopus database