Antibody Microarray Analyses of Signal Transduction Protein Expression and Phosphorylation during Porcine Oocyte Maturation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F08%3A00310174" target="_blank" >RIV/67985904:_____/08:00310174 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Antibody Microarray Analyses of Signal Transduction Protein Expression and Phosphorylation during Porcine Oocyte Maturation
Original language description
Kinex antibody microarray analyses was used to investigate the regulation of 188 protein kinases, 24 protein phosphatases, and 170 other regulatory proteins during meiotic maturation of immature germinal vesicle (GV+) pig oocytes to maturing oocytes thathad completed meiosis I (MI), and fully mature oocytes arrested at metaphase of meiosis II (MII). Western blotting analyses confirmed altered expression levels of Bub1A, IRAK4, MST2, PP4C, and Rsk2, and the phosphorylation site changes in the kinases Erk5 (T218 + Y220), FAK(S722), GSK3-beta (Y216), MEK1 (S217 + S221) and PKR1 (T451), and nucleophosmin/B23 (S4) during pig oocyte maturation.
Czech name
Analýza signálních proteinů a fosforylace v průběhu zrání prasečích oocytů s využitím protilátkových mikročipů
Czech description
Kinex protilátkové mikročipy byly použity ke sledování regulace 188 protein kináz, 24 protein fosfatáz a 170 další regulačních proteinů v průběhu zrání prasečích oocytů z GV stadia do metafáze MII. Regulace vybraných proteinů byla paralelně sledována u oocytů krav, žab a mořské hvězdice. Specifický imunoblot potvrdil změny hladin či fosforylace u Bub1A, IRAK4, MST2, PP4C, and Rsk2, Erk5 (T218 + Y220), FAK(S722), GSK3-beta (Y216), MEK1 (S217 + S221) and PKR1 (T451), and nucleophosmin/B23 (S4).
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA204%2F06%2F1297" target="_blank" >GA204/06/1297: The changes in chromatin structure during meiotic maturation of pig oocytes: correlation to chromosomal aneuploidy</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Proteome Research
ISSN
1535-3893
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
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UT code for WoS article
000257449500027
EID of the result in the Scopus database
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