Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F11%3A00359480" target="_blank" >RIV/67985904:_____/11:00359480 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma
Original language description
Serum and plasma-based biomarker discovery requires technologies with specific capabilities: sufficient proteome coverage and depth; technical reproducibly; and the scalability to enable analysis on substantial volumes of a large number of samples at reasonable cost. We have shown that plasma samples processed using ProteomeLab IgY-12 LC10 Proteome Partitioning Kits to selectively remove the 12 most highly-abundant proteins, followed by intact protein separation by two-dimensional liquid chromatography(2DLC, chromatofocusing and reversed phase) can unique enrich for middle ?lower-abundant proteins. Equally, 1DLC (reversed phase) separation of intact proteins is complementary to 2DLC. The serial use of a single piece of equipment can be prohibitively time-consuming and thus, this chapter also describes the harmonization of multiple LC instruments in order to minimize technical variation and ensure reproducibility.
Czech name
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Czech description
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Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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