Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F11%3A00364524" target="_blank" >RIV/67985904:_____/11:00364524 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.theriogenology.2010.12.019" target="_blank" >http://dx.doi.org/10.1016/j.theriogenology.2010.12.019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.theriogenology.2010.12.019" target="_blank" >10.1016/j.theriogenology.2010.12.019</a>

Result language

angličtina

Original language name

Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition

Original language description

Pre-implantation embryos derived by in vitro fertilization differ in their developmental potential from embryos obtained in vivo. In order to characterize changes in gene expression profiles caused by in vitro culture environment, we employed microarrayconstructed from bovine oocyte and preimplantation embryo-specific cDNAs (BlueChip, Universite Laval, Quebec). The analysis revealed changes in the level of 134 transcripts between in vitro derived (cultured in COOK BVC/BVB media) and in vivo derived 4-cell stage embryos and 97 transcripts were differentially expressed between 8-cell stage in vitro and in vivo embryos. The expression profiles of 7 selected transcripts (BUB3, CUL1, FBL, NOLC1, PCAF, GABPA and CNOT4) were studied in detail. We have identified a switch from Cullin 1-like transcript variant 1 to Cullin 1 transcript variant 3 (UniGene IDs BT.36789 and BT.6490, respectively) expressions around the time of bovine major gene activation (8-cell stage).

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Theriogenelogy

ISSN

0093-691X

e-ISSN

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Volume of the periodical

75

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

1582-1595

UT code for WoS article

000290925500002

EID of the result in the Scopus database

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