High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00569567" target="_blank" >RIV/67985904:_____/22:00569567 - isvavai.cz</a>
Result on the web
<a href="https://journals.biologists.com/dev/article/149/21/dev200819/280468/High-resolution-ribosome-profiling-reveals" target="_blank" >https://journals.biologists.com/dev/article/149/21/dev200819/280468/High-resolution-ribosome-profiling-reveals</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1242/dev.200819" target="_blank" >10.1242/dev.200819</a>
Alternative languages
Result language
angličtina
Original language name
High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development
Original language description
High-resolution ribosome fractionation and low-input ribosome profiling of bovine oocytes and preimplantation embryos has enabled us to define the translational landscapes of early embryo development at an unprecedented level. We analyzed the transcriptome and the polysome-and non-polysome-bound RNA profiles of bovine oocytes (germinal vesicle and metaphase II stages) and early embryos at the two-cell, eight-cell, morula and blastocyst stages, and revealed four modes of translational selectivity: (1) selective translation of non-abundant mRNAs, (2) active, but modest translation of a selection of highly expressed mRNAs, (3) translationally suppressed abundant to moderately abundant mRNAs, and (4) mRNAs associated specifically with monosomes. A strong translational selection of low-abundance transcripts involved in metabolic pathways and lysosomes was found throughout bovine embryonic development. Notably, genes involved in mitochondrial function were prioritized for translation. We found that translation largely reflected transcription in oocytes and two-cell embryos, but observed a marked shift in the translational control in eight-cell embryos that was associated with the main phase of embryonic genome activation. Subsequently, transcription and translation become more synchronized in morulae and blastocysts. Taken together, these data reveal a unique spatiotemporal translational regulation that accompanies bovine preimplantation development.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
<a href="/en/project/GA22-27301S" target="_blank" >GA22-27301S: From meiosis to mitosis - is starting a new life under in vitro conditions equivalent to that in vivo?</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Development
ISSN
0950-1991
e-ISSN
1477-9129
Volume of the periodical
149
Issue of the periodical within the volume
21
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
dev200819
UT code for WoS article
000903918600004
EID of the result in the Scopus database
2-s2.0-85141889757