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High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00569567" target="_blank" >RIV/67985904:_____/22:00569567 - isvavai.cz</a>

  • Result on the web

    <a href="https://journals.biologists.com/dev/article/149/21/dev200819/280468/High-resolution-ribosome-profiling-reveals" target="_blank" >https://journals.biologists.com/dev/article/149/21/dev200819/280468/High-resolution-ribosome-profiling-reveals</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1242/dev.200819" target="_blank" >10.1242/dev.200819</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development

  • Original language description

    High-resolution ribosome fractionation and low-input ribosome profiling of bovine oocytes and preimplantation embryos has enabled us to define the translational landscapes of early embryo development at an unprecedented level. We analyzed the transcriptome and the polysome-and non-polysome-bound RNA profiles of bovine oocytes (germinal vesicle and metaphase II stages) and early embryos at the two-cell, eight-cell, morula and blastocyst stages, and revealed four modes of translational selectivity: (1) selective translation of non-abundant mRNAs, (2) active, but modest translation of a selection of highly expressed mRNAs, (3) translationally suppressed abundant to moderately abundant mRNAs, and (4) mRNAs associated specifically with monosomes. A strong translational selection of low-abundance transcripts involved in metabolic pathways and lysosomes was found throughout bovine embryonic development. Notably, genes involved in mitochondrial function were prioritized for translation. We found that translation largely reflected transcription in oocytes and two-cell embryos, but observed a marked shift in the translational control in eight-cell embryos that was associated with the main phase of embryonic genome activation. Subsequently, transcription and translation become more synchronized in morulae and blastocysts. Taken together, these data reveal a unique spatiotemporal translational regulation that accompanies bovine preimplantation development.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10605 - Developmental biology

Result continuities

  • Project

    <a href="/en/project/GA22-27301S" target="_blank" >GA22-27301S: From meiosis to mitosis - is starting a new life under in vitro conditions equivalent to that in vivo?</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Development

  • ISSN

    0950-1991

  • e-ISSN

    1477-9129

  • Volume of the periodical

    149

  • Issue of the periodical within the volume

    21

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    15

  • Pages from-to

    dev200819

  • UT code for WoS article

    000903918600004

  • EID of the result in the Scopus database

    2-s2.0-85141889757