Single Molecule RNA Localization and Translation in the Mammalian Oocyte and Embryo

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00546076" target="_blank" >RIV/67985904:_____/21:00546076 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S002228362100396X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S002228362100396X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jmb.2021.167166" target="_blank" >10.1016/j.jmb.2021.167166</a>

Result language
angličtina
Original language name
Single Molecule RNA Localization and Translation in the Mammalian Oocyte and Embryo
Original language description
During oocyte growth the cell accumulates RNAs to contribute to oocyte and embryo development which progresses with ceased transcription. To investigate the subcellular distribution of specific RNAs and their translation we developed a technique revealing several instances of localized translation with distinctive regulatory implications. We analyzed the localization and expression of candidate non-coding and mRNAs in the mouse oocyte and embryo. Furthermore, we established simultaneous visualization of mRNA and in situ translation events validated with polysomal occupancy. We discovered that translationally dormant and abundant mRNAs CyclinB1 and Mos are localized in the cytoplasm of the fully grown GV oocyte forming cloud-like structures with consequent abundant translation at the center of the MII oocyte. Coupling detection of the localization of specific single mRNA molecules with their translation at the subcellular context is a valuable tool to quantitatively study temporal and spatial translation of specific target mRNAs to understand molecular processes in the developing cell.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology

Project
<a href="/en/project/GA18-19395S" target="_blank" >GA18-19395S: Non-coding RNAs matters: role in mammalian oocyte and embryo</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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