Bovine preimplantation embryos with silenced nucleophosmin mRNA are able to develop until the blastocyst stage

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F12%3A00386816" target="_blank" >RIV/67985904:_____/12:00386816 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1530/REP-12-0033" target="_blank" >http://dx.doi.org/10.1530/REP-12-0033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1530/REP-12-0033" target="_blank" >10.1530/REP-12-0033</a>

Result language

angličtina

Original language name

Bovine preimplantation embryos with silenced nucleophosmin mRNA are able to develop until the blastocyst stage

Original language description

This study was conducted to investigate the effect of silencing nucleophosmin in the development of in vitro-produced bovine embryos. Nucleophosmin is an abundant multifunctional nucleolar phosphoprotein that participates, for example, in ribosome biogenesis or centrosome duplication control. We showed that although the transcription of embryonic nucleophosmin started already at late eight-cell stage, maternal protein was stored throughout the whole preimplantation development and was sufficient for theprogression to the blastocyst stage. At the beginning of embryogenesis, translation occurs on maternally derived ribosomes, the functionally active nucleoli emerge during the fourth cell cycle in bovines. We found that nucleophosmin localisation reflected the nucleolar formation during bovine preimplantation development. The protein was detectable from the beginning of embryonic development. Before embryonic genome activation, it was dispersed throughout the nucleoplasm. The typical nuc

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Reproduction

ISSN

1470-1626

e-ISSN

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Volume of the periodical

144

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

349-359

UT code for WoS article

000308071300006

EID of the result in the Scopus database

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