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The embryonic nucleologenesis during inhibition of major transcriptional activity in bovine preimplantation embryos

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F12%3A00390776" target="_blank" >RIV/67985904:_____/12:00390776 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.2478/s11756-012-0066-1" target="_blank" >http://dx.doi.org/10.2478/s11756-012-0066-1</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.2478/s11756-012-0066-1" target="_blank" >10.2478/s11756-012-0066-1</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The embryonic nucleologenesis during inhibition of major transcriptional activity in bovine preimplantation embryos

  • Original language description

    Common features of embryonic genome activation in mammalian and non-mammalian embryos are the colocalization of pre-assembled complexes of maternally inherited nucleolar proteins, the so-called nucleolus precursor bodies and de novo synthesized transcripts with ribosomal DNA. The de novo transcription of messenger RNA and ribosomal RNA proteins is required for the development of functional nuclei during the major activation of the embryonic genome. The aim of our work was to investigate to what extent.Autoradiography and transmission electron microscopy has been applied in in vitro produced bovine embryos. The embryos were cultured to the late 8-cell stage with: alpha-amanitin; a specific inhibitor of RNA-polymerases II and III transcription; actinomycin D; a specific inhibitor of RNA polymerase I transcription; and without inhibitors (control group). Nucleoplasm and nucleolar structures displayed strong autoradiographic labeling and showed the initial development of fibrillo-granular

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biologia

  • ISSN

    0006-3088

  • e-ISSN

  • Volume of the periodical

    67

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    SK - SLOVAKIA

  • Number of pages

    8

  • Pages from-to

    818-825

  • UT code for WoS article

    000305840100026

  • EID of the result in the Scopus database