Inhibition of proteasomal proteolysis affects expression of extracellular matrix components and steroidogenesis in porcine oocyte-cumulus complexes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F12%3A00371581" target="_blank" >RIV/67985904:_____/12:00371581 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.domaniend.2011.09.003" target="_blank" >http://dx.doi.org/10.1016/j.domaniend.2011.09.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.domaniend.2011.09.003" target="_blank" >10.1016/j.domaniend.2011.09.003</a>

Result language

angličtina

Original language name

Inhibition of proteasomal proteolysis affects expression of extracellular matrix components and steroidogenesis in porcine oocyte-cumulus complexes

Original language description

Porcine oocyte-cumulus complexes (OCCs) form an expanded cumulus extracellular matrix (ECM) in response to gonadotropins during meiotic maturation. Essential components of ECM are hyaluronan (HA), tumor necrosis factor a-induced protein 6 (TNFAIP6) and heavy chains (HC) of interalpha-trypsin inhibitor. To form expanded cumulus ECM, intermediate complexes (TNFAIP6-HC) must bind to HA to allow HC transfer onto HA. Protein turnover by the ubiquitin-proteasome pathway is poorly characterized in this process. It is known that the specific proteasomal inhibitor MG132 prevents cumulus expansion and formation of ECM. To determine whether inhibition of proteasomal proteolysis with MG132 affects cumulus cell steroidogenesis and expression of the cumulus expansion-related components (hyaluronan synthase type 2, HAS2, TNFAIP6) we cultured porcine OCCs and granulosa cells (GCs) in a medium supplemented with FSH/LH.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/GAP502%2F11%2F0593" target="_blank" >GAP502/11/0593: Signaling pathways and genes regulating gonadotropin-induced maturation of pig cumulus-oocyte complexes</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Domestic Animal Endocrinology

ISSN

0739-7240

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

50-62

UT code for WoS article

000298443900006

EID of the result in the Scopus database

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