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Synthesis of Tumor Necrosis Factor Alpha Induced Protein 6 in Porcine Preovulatory Follicles; A Study with A38 Antibody

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F08%3A00306895" target="_blank" >RIV/67985904:_____/08:00306895 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Synthesis of Tumor Necrosis Factor Alpha Induced Protein 6 in Porcine Preovulatory Follicles; A Study with A38 Antibody

  • Original language description

    Our results, together with the high sequence similarity found among human, rabbit, mouse, rat, bovine and equine TNFAIP6 and the expression of this protein and/or the respective gene in preovulatory follicles of all the examined species, strongly supportthe concept that TNFAIP6-mediated HC covalent transfer onto hyaluronic acid (HA) synthesized by cumulus cells is a mechanism common to mammalian OCCs. However, in contrast to the situation observed in the mouse, anti-TNFAIP6 A38 antibody did not preventcumulus expansion nor HA accumulation in the matrix of pig OCCs stimulated with FSH. It suggests that porcine OCCs have evolved supplemental mechanisms for holding HA in the matrix. Thus, it is reasonable to hypothesize that different relative levels ofHA, TNFAIP6, versican as well as other matrix components are synthesized by cumulus cells of different species, with the aim to create for each species the matrix that is the most suitable for ovulation and oocyte fertilization.

  • Czech name

    Syntéza TNFAIP6 v předovulačních folikulech prasat. Sledování vlivu protilátky A38

  • Czech description

    Naše výsledky, zároveň s vysokou podobností sekvence, která byla zjištěna mezi TNFAIP6 u lidí, králíků, myší, krys, krav a koní a exprese tohoto proteinu a/anebo genu v preovulačním folikulu všech těchto zvířat silně podporuji koncept, že TNFAIP6-zprostředkovaný kovalentní transfer těžkých řetězců (HC) k hyaluronové kyselině (HA) , která je syntetizována buňkami kumulu, je mechanizmus společný pro OCCs savců. Ačkoliv, na rozdíl od situace u myší, anti-TNFAIP6 A38 protilátka nezabránila ani expanzi kumulu ani akumulaci hyaluronové kyseliny v matrix u OCCs prasat po FSH stimulaci. To naznačuje, že u OCC prasat se vyvinuly přídavné mechanizmy pro akumulaci a organizaci HA do matrix. Proto se můžeme domnívat, že rozdílné hladiny HA, TNFAIP6, versicanu, tenascinu jako i dalších složek jsou syntetizovány buňkami kumulu různých druhů zvířat s cílem vytvořit pro každý druh matrix, který usnadní ovulaci a oplození oocytu.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA305%2F05%2F0960" target="_blank" >GA305/05/0960: Immunodetection of structural constituents in cumulus -extracellular matrix, that are crucial for female fertility.</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biology of Reproduction

  • ISSN

    0006-3363

  • e-ISSN

  • Volume of the periodical

    78

  • Issue of the periodical within the volume

    -

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

    903-909

  • UT code for WoS article

  • EID of the result in the Scopus database