Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F13%3A00397823" target="_blank" >RIV/67985904:_____/13:00397823 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.fertnstert.2012.12.040" target="_blank" >http://dx.doi.org/10.1016/j.fertnstert.2012.12.040</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fertnstert.2012.12.040" target="_blank" >10.1016/j.fertnstert.2012.12.040</a>

Result language
angličtina
Original language name
Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium
Original language description
Objective: To determine whether inhibition of epidermal growth factor (EGF) receptor tyrosine kinase with lapatinib affects oocyte maturation, expression of the cumulus expansion-associated genes such as tumor necrosis factor alpha-induced protein 6 (TNFAIP6) and prostaglandin-endoperoxide synthase 2 (PTGS2), and synthesis of hyaluronan (HA) and progesterone (P) by porcine oocyte cumulus complexes (OCC). Design: Our work focuses on lapatinib, an orally active small molecule that selectively inhibits thetyrosine kinase domain of both EGF receptor and human EGF receptor 2, and downstream signaling. Setting: A reproductive biology laboratory. Patient(s): Not applicable. Intervention(s): Porcine OCC were cultured in vitro in a medium with FSH/LH in the presence/absence of lapatinib. Main Outcome Measure(s): Methods performed: real-time reverse transcriptase-polymerase chain reaction (PCR), immunofluorescence, RIA. Result(s): In FSH/LH-stimulated and expanded cumulus oophorus extracellular
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Project
<a href="/en/project/GAP502%2F11%2F0593" target="_blank" >GAP502/11/0593: Signaling pathways and genes regulating gonadotropin-induced maturation of pig cumulus-oocyte complexes</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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