Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F14%3A00427357" target="_blank" >RIV/67985904:_____/14:00427357 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.domaniend.2014.01.003" target="_blank" >http://dx.doi.org/10.1016/j.domaniend.2014.01.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.domaniend.2014.01.003" target="_blank" >10.1016/j.domaniend.2014.01.003</a>

Result language

angličtina

Original language name

Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes

Original language description

This study was designed to determine whether inhibition of either cyclooxygenase-2 (COX-2) by indomethacin or progesterone receptor (PR) by PR antagonist, RU486, affects oocyte maturation, progesterone production, and covalent binding between hyaluronan(HA) and heavy chains of inter-? trypsin inhibitor, as well as expression of cumulus expansion-associated proteins (HA-binding protein, tumor necrosis factor ?-induced protein 6, pentraxin 3) in oocyte-cumulus complexes (OCCs). The experiments were basedon freshly isolated porcine OCC cultures in which the consequences of PR and COX-2 inhibition on the final processes of oocyte maturation were determined. Granulosa cells (GCs) and OCCs were cultured in medium supplemented with FSH/LH (both 100ng/mL) inthe presence/absence of RU486 or indomethacin. Western blot analysis, 3H-glucosamine hydrochloride assay, immunofluorescence, and radioimmunoassay were performed. Only treatment with RU486 (25 ?M) caused a decrease in the number of oocyt

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/GAP502%2F11%2F0593" target="_blank" >GAP502/11/0593: Signaling pathways and genes regulating gonadotropin-induced maturation of pig cumulus-oocyte complexes</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Domestic Animal Endocrinology

ISSN

0739-7240

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

7-14

UT code for WoS article

000337643900002

EID of the result in the Scopus database

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