Senzitive method of Caspase-3 detection in single stem cell
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F13%3A00420849" target="_blank" >RIV/67985904:_____/13:00420849 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/13:00420849
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Senzitive method of Caspase-3 detection in single stem cell
Original language description
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10?15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Chemické Listy. Roč. 107, Issue s3.
ISBN
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ISSN
1213-7103
e-ISSN
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Number of pages
2
Pages from-to
289-290
Publisher name
Česká společnost chemická
Place of publication
Praha
Event location
Brno
Event date
Nov 12, 2013
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000328730800002