Bioluminescence determination of active caspase-3 in single apoptotic cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F13%3A00387334" target="_blank" >RIV/67985904:_____/13:00387334 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/13:00387334 RIV/62157124:16170/13:43871765
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201200675" target="_blank" >http://dx.doi.org/10.1002/elps.201200675</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201200675" target="_blank" >10.1002/elps.201200675</a>

Result language
angličtina
Original language name
Bioluminescence determination of active caspase-3 in single apoptotic cells
Original language description
In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. The technology is based on the specific cleavage of modified luciferin by caspase-3, an immediate bioluminescence reaction of free luciferin with luciferase followed by emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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