Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F16%3A00467739" target="_blank" >RIV/68081715:_____/16:00467739 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Original language description
Caspases are proteases that play key role in the process of apoptosis, the programmedncell death. Among them, caspase-3 and -7 are main executioner caspases that cleavenmany vital proteins during apoptosis and after their widespread activation, the processncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturizedndevice for parallel analysis of eight samples was developed. The assay is based on thenmodified luciferin-firefly luciferase bioluminescence (BL) system. Individualnsuspended cells were collected and transferred into detection microvials using anmicromanipulator. The bioluminescence was detected using a photon counting headnwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in bothnapoptotic and non-apoptotic cells was reached.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA14-28254S" target="_blank" >GA14-28254S: Single cell analysis of signaling molecules</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis
ISBN
978-80-904959-4-4
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
170-173
Publisher name
Ústav analytické chemie AV ČR, v. v. i.
Place of publication
Brno
Event location
Brno
Event date
Oct 17, 2016
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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