A miniaturized device for bioluminescence analysis of caspase-3/7 activity in a single apoptotic cell

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F14%3A%230000552" target="_blank" >RIV/00209805:_____/14:#0000552 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/14:00437556 RIV/62157124:16170/14:43872987

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs00216-014-7949-7" target="_blank" >http://link.springer.com/article/10.1007%2Fs00216-014-7949-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-014-7949-7" target="_blank" >10.1007/s00216-014-7949-7</a>

Result language

angličtina

Original language name

A miniaturized device for bioluminescence analysis of caspase-3/7 activity in a single apoptotic cell

Original language description

Caspases are key enzymes activated during the apoptotic machinery. Apoptosis as a way of programmed cell death becomes deregulated in some pathologies including cancer transformations, neurodegenerative, or autoimmune diseases. Most of the methods available for the detection of apoptosis and caspases provide qualitative information only or quantification data as an average from cell populations or cell lysates. Several reports point to the importance of more accurate single-cell analyses in biomedical studies due to heterogeneity at tissue as well as cell level. To meet these requirements, we developed a miniaturized device enabling detection and quantification of active caspase-3/7 in individual cells at a femtogram level (10-15 g). The active caspase-3/7 detection protocol is based on the bioluminescence chemistry commercially available as a Caspase-GloTM 3/7 reagent developed by Promega. As a model, we used human stem cells treated by camptothecin to induce apoptosis. Individual apo

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and bioanalytical chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

406

Issue of the periodical within the volume

22

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

5389-5394

UT code for WoS article

000340877200017

EID of the result in the Scopus database

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