A frame-supported ultrathin electrospun polymer membrane for transplantation of retinal pigment epithelial cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F15%3A00446658" target="_blank" >RIV/67985904:_____/15:00446658 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/15:00446658 RIV/00216208:11110/15:10297879 RIV/00216208:11120/15:43910261
Result on the web
<a href="http://dx.doi.org/10.1088/1748-6041/10/4/045022" target="_blank" >http://dx.doi.org/10.1088/1748-6041/10/4/045022</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1088/1748-6041/10/4/045022" target="_blank" >10.1088/1748-6041/10/4/045022</a>
Alternative languages
Result language
angličtina
Original language name
A frame-supported ultrathin electrospun polymer membrane for transplantation of retinal pigment epithelial cells
Original language description
We report on the design and fabrication of a frame-supported nanofibrous membrane for the transplantation of retinal pigment epithelial (RPE) cells, which is a promising therapeutic option for the treatment of degenerative retinal disorders. The membranous cell carrier prepared from 640 nm-thick poly(DL-lactide) fibres uniquely combines high porosity, large pore size and low thickness, to maximize the nutrient supply to the transplanted cells in the subretinal space and thus to enhance the therapeutic effect of the transplantation. The carrier was prepared by electrospinning, which made it easy to embed a 95 μm-thick circular supporting frame 2 mm in diameter. Implantations into enucleated porcine eyes showed that the frame enabled the ultrathin membrane to be handled without irreversible folding, and allowed the membrane to regain its flat shape when inserted into the subretinal space. We further demonstrated that the minimum membrane thickness compatible with the surgical procedure and instrumentation employed here was as low as 4 μm. Primary porcine RPE cells cultivated on the membranes formed a confluent monolayer, expressed RPE-specific differentiation markers and showed transepithelial resistance close to that of the native RPE. Most importantly, the majority of the RPE cells transplanted into the subretinal space remained viable. The ultrathin, highly porous, and surgically convenient cell carrier presented here has the potential to improve the integration and the functionality of transplanted RPE cells.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomedical Materials
ISSN
1748-6041
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
4
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
"045022_1"-"045022_15"
UT code for WoS article
000360215600023
EID of the result in the Scopus database
2-s2.0-84982810079