Subretinal implantation of RPE on a carrier in minipigs: guidelines for preoperative preparations, surgical techniques, and postoperative care
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00565231" target="_blank" >RIV/67985904:_____/22:00565231 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/22:00565231 RIV/68378041:_____/22:00565231 RIV/00064173:_____/22:43924017 RIV/00216208:11120/22:43924017 RIV/00216208:11310/22:10448505
Result on the web
<a href="https://review.jove.com/t/63505/subretinal-implantation-rpe-on-carrier-minipigs-guidelines-for" target="_blank" >https://review.jove.com/t/63505/subretinal-implantation-rpe-on-carrier-minipigs-guidelines-for</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/63505" target="_blank" >10.3791/63505</a>
Alternative languages
Result language
angličtina
Original language name
Subretinal implantation of RPE on a carrier in minipigs: guidelines for preoperative preparations, surgical techniques, and postoperative care
Original language description
Degenerative disorders of the retina (including age-related macular degeneration), which originate primarily at or within the retinal pigmented epithelial (RPE) layer, lead to a progressive disorganization of the retinal anatomy and the deterioration of visual function. The substitution of damaged RPE cells (RPEs) with in vitro cultured RPE cells using a subretinal cell carrier has shown potential for re-establishing the anatomical structure of the outer retinal layers and is, therefore, being further studied. Here, we present the principles of a surgical technique that allows for the effective subretinal transplantation of a cell carrier with cultivated RPEs into minipigs. The surgeries were performed under general anesthesia and included a standard lens-sparing three-port pars plana vitrectomy (PPV), subretinal application of a balanced salt solution (BSS), a 2.7 mm retinotomy, implantation of a nanofibrous cell carrier into the subretinal space through an additional 3.0 mm sclerotomy, fluid-air exchange (FAX), silicone oil tamponade, and closure of all the sclerotomies. This surgical approach was used in 29 surgeries (18 animals) over the past 8 years with a success rate of 93.1%. Anatomic verification of the surgical placement was carried out using in vivo fundus imaging (fundus photography and optical coherence tomography). The recommended surgical steps for the subretinal implantation of RPEs on a carrier in minipig eyes can be used in future preclinical studies using large-eye animal models.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30207 - Ophthalmology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Jove-Journal of Visualized Experiments
ISSN
1940-087X
e-ISSN
—
Volume of the periodical
2022
Issue of the periodical within the volume
189
Country of publishing house
US - UNITED STATES
Number of pages
26
Pages from-to
e63505
UT code for WoS article
000911862800005
EID of the result in the Scopus database
2-s2.0-85142851944