Feasibility of Direct Vitrectomy-Sparing Subretinal Injection for Gene Delivery in Large Animals
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00587897" target="_blank" >RIV/67985904:_____/24:00587897 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/24:43926984 RIV/00064173:_____/24:43926984
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/02713683.2024.2343335" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/02713683.2024.2343335</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/02713683.2024.2343335" target="_blank" >10.1080/02713683.2024.2343335</a>
Alternative languages
Result language
angličtina
Original language name
Feasibility of Direct Vitrectomy-Sparing Subretinal Injection for Gene Delivery in Large Animals
Original language description
PurposeTo assess the safety and feasibility of direct vitrectomy-sparing subretinal injection for gene delivery in a large animal model. Methods: The experimental Lib & ecaron, chov minipigs were used for subretinal delivery of a plasmid DNA vector (pS/MAR-CMV-copGFP) with cytomegalovirus (CMV) promoter, green fluorescent protein (GFP) reporter (copGFP) and a scaffold/matrix attachment region (S/MAR) sequence. The eyes were randomized to subretinal injection of the vector following pars plana vitrectomy (control group) or a direct injection without prior vitrectomy surgery (experimental group). Intra- and post-operative observations up to 30 days after surgery were compared.ResultsSix eyes of three mini-pigs underwent surgery for delivery into the subretinal space. Two eyes in the control group were operated with a classical approach (lens-sparing vitrectomy and posterior hyaloid detachment). The other four eyes in the experimental group were injected directly with a subretinal cannula without vitrectomy surgery. No adverse events, such as endophthalmitis, retinal detachment and intraocular pressure elevation were observed post-operatively. The eyes in the experimental group had both shorter surgical time and recovery while achieving the same surgical goal. Conclusions: This pilot study demonstrates that successful subretinal delivery of gene therapy vectors is achievable using a direct injection without prior vitrectomy surgery.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Result continuities
Project
<a href="/en/project/EH22_008%2F0004562" target="_blank" >EH22_008/0004562: Excellent Research in Regenerative Medicine</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Eye Research
ISSN
0271-3683
e-ISSN
1460-2202
Volume of the periodical
49
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
879-887
UT code for WoS article
001209142800001
EID of the result in the Scopus database
2-s2.0-85191288929