Optimizing Subretinal Bleb Formation for Visual Streak Involvement in a Porcine Model for Retinal Gene Therapy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00600821" target="_blank" >RIV/67985904:_____/24:00600821 - isvavai.cz</a>
Result on the web
<a href="https://iovs.arvojournals.org/article.aspx?articleid=2802174" target="_blank" >https://iovs.arvojournals.org/article.aspx?articleid=2802174</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1167/iovs.65.12.41" target="_blank" >10.1167/iovs.65.12.41</a>
Alternative languages
Result language
angličtina
Original language name
Optimizing Subretinal Bleb Formation for Visual Streak Involvement in a Porcine Model for Retinal Gene Therapy
Original language description
PURPOSE. Subretinal (SR) injection in porcine models is a promising avenue for preclinical evaluation of cell and gene therapies. Targeting of the subretinal fluid compartment (bleb) is critical to the procedure, especially if treatment of the cone-rich area centralis is required (i.e., visual streak [VS] in pigs). To our knowledge, this study is the first to investigate the influence of injection site placement on VS involvement in the pig eye. METHODS. We performed 23-gauge pars plana vitrectomy followed by SR injection in 41 eyes of 21 animals (Sus scrofa domesticus). In 27 eyes (65.9%), the injection site was placed superior to the VS, and in 14 eyes (34.1%) it was placed inferior to it. Using intraoperative imaging, blebs were classified based on their propagation behavior relative to the VS. RESULTS. In 79% of cases, blebs from inferior injection sites developed away from the VS, exhibiting a mean +/- SEM vertical anisotropy (AP) of 0.67 +/- 0.11. In contrast, blebs from superior injection sites tended to develop toward the VS with an AP of 1.27 +/- 0.18 (P = 0.0070). Blebs developed away from the VS in only 41% of injections (P = 0.0212). Inferior blebs were orientated close to 0 degrees (horizontal), whereas superior blebs displayed varied orientations with a mean angle of 56 degrees (P = 0.0008). CONCLUSIONS. Bleb propagation was anisotropic (i.e., directionally biased) and dependent on injection site placement. Superior injection sites led to superior VS detachment. Morphological analysis suggested increased adhesion forces at the VS and superior vascular arcades. This study will aid the planning of surgeries for targeted subretinal delivery in pig models.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Result continuities
Project
<a href="/en/project/EH22_008%2F0004562" target="_blank" >EH22_008/0004562: Excellent Research in Regenerative Medicine</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Investigative Ophthalmology and Visual Science
ISSN
0146-0404
e-ISSN
1552-5783
Volume of the periodical
65
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
41
UT code for WoS article
001345204800003
EID of the result in the Scopus database
2-s2.0-85208001379