31P MR Spectroscopy of the Testes and Immunohistochemical Analysis of Sperm 
of Transgenic Boars Carried N terminal Part of Human Mutated Huntingtin

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F15%3A00476983" target="_blank" >RIV/67985904:_____/15:00476983 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/15:00452777 RIV/00023001:_____/15:00059489

Result on the web

<a href="http://dx.doi.org/10.14735/amcsnn20152S28" target="_blank" >http://dx.doi.org/10.14735/amcsnn20152S28</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14735/amcsnn20152S28" target="_blank" >10.14735/amcsnn20152S28</a>

Result language

angličtina

Original language name

31P MR Spectroscopy of the Testes and Immunohistochemical Analysis of Sperm 
of Transgenic Boars Carried N terminal Part of Human Mutated Huntingtin

Original language description

Huntington’s disease (HD) is an inherited autosomal neurodegenerative disorder characterized by motor dysfunctions, behavioral and cognitive disturbances. It affects predominantly the brain, however, changes were found also in peripheral tissues. Some of these changes can result from direct expression of mutant huntingtin; its highest levels have been found in the brain and testes. In 2009 we established a minipig model of HD (TgHD) expressing N terminal (548aa) part of human mutated huntingtin encoded 124 CAG/ CAA repeats. Previous research has revealed the presence of reduced fertility and fewer spermatozoa per ejaculate in TgHD boars started at 13 months of age. The aim of this study was to determine changes in the testes of 24 months old transgenic boars (F2 generation in vivo) using non invasive methodology of 31P magnetic resonance (MR) spectroscopy as well as to perform imunohistochemical analysis of TgHD sperm collected fromform F1 and F3 generation before HD onset. The results have shown significant reduction of relative phosphodiester concentration in testicular parenchyma of TgHD boars compared to wild type (WT) ones of the same ages. Moreover immunohistochemical analysis of sperm collected from TgHD and WT have revealed exclusive anti polyQ specific (clone 3B5H10) as well as significantly increased anti huntingtin (clone EPR5526) staining in transgenic spermatozoa tails in comparison with WT counterparts. Thus, our results are suggestive of the negative impact of human mutated huntingtin on testes metabolism as well as sperm abnormalities.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Česká a Slovenská neurologie a neurochirurgie

ISSN

1210-7859

e-ISSN

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Volume of the periodical

78

Issue of the periodical within the volume

Suppl 2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

28-33

UT code for WoS article

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EID of the result in the Scopus database

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