Chalcone derivatives cause accumulation of colon cancer cells in the G2/M phase and induce apoptosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F16%3A00458508" target="_blank" >RIV/67985904:_____/16:00458508 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.lfs.2016.02.073" target="_blank" >http://dx.doi.org/10.1016/j.lfs.2016.02.073</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.lfs.2016.02.073" target="_blank" >10.1016/j.lfs.2016.02.073</a>
Alternative languages
Result language
angličtina
Original language name
Chalcone derivatives cause accumulation of colon cancer cells in the G2/M phase and induce apoptosis
Original language description
Aims: Chalcones, naturally occurring open-chain polyphenols abundant in plants, have demonstrated antiproliferative activity in several cancer cell lines. In the present study, the potential anticancer activity of two synthetic analogues named Ch1 and Ch2 in colon cancer cell line was investigated. nMain methods: Antiproliferative activities of both synthetic analogues were assessed by Growth Inhibition Assay (MTT) and xCELLigence cell analysis. Apoptosis was assessed by annexin V/PI staining (early stage) or by DNA fragmentation (final stage). To study the cell death mechanism induced by tested substances, we assessed a series of assays including measurements of the caspase 3 activity, membrane mitochondrial potential (MMP) changes, reactive oxygen species (ROS) production by flow cytometry and expression of important apoptosis-related genes by realtime PCR. nKey findings: We found concentration and time-dependent cytotoxicity, inhibition of proliferation of Caco-2 cells after Ch1 and Ch2 treatment in parallel with G2/M phase cell cycle arrest and increased cell proportion in subG0/G1 population with annexin V positivity. We demonstrated that both Ch1 and Ch2 induced caspase-dependent cell death associated with increased ROS production, suppressed Bcl-2 and Bcl-xL and enhanced Bax expression. Treatment of Ch1 also suppressed alpha-, alpha- and beta 5-tubulins, on the other hand Ch2 only suppressed alpha-tubulin expression. nSignificance: Presented chalcones induce apoptosis by intrinsic pathways, and therefore may be an interesting strategy for cancer therapy.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Life Sciences
ISSN
0024-3205
e-ISSN
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Volume of the periodical
150
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
32-38
UT code for WoS article
000371790700005
EID of the result in the Scopus database
2-s2.0-84978523901