Haspin kinase regulates microtubule-organizing center clustering and stability through Aurora kinase C in mouse oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F16%3A00468636" target="_blank" >RIV/67985904:_____/16:00468636 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1242/jcs.189340" target="_blank" >http://dx.doi.org/10.1242/jcs.189340</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jcs.189340" target="_blank" >10.1242/jcs.189340</a>

Result language

angličtina

Original language name

Haspin kinase regulates microtubule-organizing center clustering and stability through Aurora kinase C in mouse oocytes

Original language description

Meiotic oocytes lack classic centrosomes and, therefore, bipolar spindle assembly depends on clustering of acentriolar microtubule-organizing centers (MTOCs) into two poles. However, the molecular mechanism regulating MTOC assembly into two poles is not fully understood. The kinase haspin (also known as GSG2) is required to regulate Aurora kinase C (AURKC) localization at chromosomes during meiosis I. Here, we show that inhibition of haspin perturbed MTOC clustering into two poles and the stability of the clustered MTOCs. Furthermore, we show that AURKC localizes to MTOCs in mouse oocytes. Inhibition of haspin perturbed the localization of AURKC at MTOCs, and overexpression of AURKC rescued the MTOC-clustering defects in haspin-inhibited oocytes. Taken together, our data uncover a role for haspin as a regulator of bipolar spindle assembly by regulating AURKC function at acentriolar MTOCs in oocytes.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LO1609" target="_blank" >LO1609: Models of the Serious Human Diseases: Traumatic Spinal Cord Injury, Huntington’s Disease, Melanoma and Infertility</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Science

ISSN

0021-9533

e-ISSN

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Volume of the periodical

129

Issue of the periodical within the volume

19

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

3648-3660

UT code for WoS article

000389594700013

EID of the result in the Scopus database

2-s2.0-84995484441