Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F17%3A00472227" target="_blank" >RIV/67985904:_____/17:00472227 - isvavai.cz</a>

Alternative codes found

RIV/68407700:21230/17:00307540 RIV/68407700:21730/17:00307540 RIV/00216224:14310/17:00096114 RIV/62156489:43210/17:43910835 RIV/00216305:26620/17:PU122784

Result on the web

<a href="http://dx.doi.org/10.1038/srep40169" target="_blank" >http://dx.doi.org/10.1038/srep40169</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/srep40169" target="_blank" >10.1038/srep40169</a>

Result language

angličtina

Original language name

Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

Original language description

We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10609 - Biochemical research methods

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

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UT code for WoS article

000392234200001

EID of the result in the Scopus database

2-s2.0-85009230317