Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F17%3A00485263" target="_blank" >RIV/67985904:_____/17:00485263 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/17:75088

Result on the web

<a href="http://dx.doi.org/10.3390/ijms18122697" target="_blank" >http://dx.doi.org/10.3390/ijms18122697</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms18122697" target="_blank" >10.3390/ijms18122697</a>

Result language

angličtina

Original language name

Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research

Original language description

Melanoma is a skin cancer with permanently increasing incidence and resistance to therapies in advanced stages. Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. Cytokines are key factors regulating immune response and intercellular communication in tumour microenvironment. Cytokines may be used in therapy of melanoma to modulate immune response. Cytokines also possess diagnostic and prognostic potential and cytokine production may reflect effects of immunotherapies. The purpose of this review is to give an overview of recent advances in proteomic techniques for the detection and quantification of cytokines in melanoma research. Approaches covered span from mass spectrometry to immunoassays for single molecule detection (ELISA, western blot), multiplex assays (chemiluminescent, bead-based (Luminex) and planar antibody arrays), ultrasensitive techniques (Singulex, Simoa, immuno-PCR, proximity ligation/extension assay, immunomagnetic reduction assay), to analyses of single cells producing cytokines (ELISpot, flow cytometry, mass cytometry and emerging techniques for single cell secretomics). Although this review is focused mainly on cancer and particularly melanoma, the discussed techniques are in general applicable to broad research field of biology and medicine, including stem cells, development, aging, immunology and intercellular communication.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10609 - Biochemical research methods

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

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Volume of the periodical

18

Issue of the periodical within the volume

12

Country of publishing house

CH - SWITZERLAND

Number of pages

32

Pages from-to

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UT code for WoS article

000418896700199

EID of the result in the Scopus database

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