In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F19%3A00506137" target="_blank" >RIV/67985904:_____/19:00506137 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/19:80268 RIV/00027014:_____/19:N0000077
Result on the web
<a href="https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1490-y" target="_blank" >https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1490-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12866-019-1490-y" target="_blank" >10.1186/s12866-019-1490-y</a>
Alternative languages
Result language
angličtina
Original language name
In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time
Original language description
BackgroundKnowledge of microbiota composition, persistence, and transmission as well as the overall function of the bacterial community is important and may be linked to honey bee health. This study aimed to investigate the inter-individual variation in the gut microbiota in honey bee larvae and pupae.ResultsIndividual larvae differed in the composition of major bacterial groups. In the majority of 5th instar bees, Firmicutes showed predominance (70%), however, after larval defecation and during pupation, the abundance decreased to 40%, in favour of Gammaproteobacteria. The 5th instar larvae hosted significantly more (P<0.001) Firmicutes than black pupae. Power calculations revealed that 11 and 18 replicate-individuals, respectively, were required for the detection of significant differences (P<0.05) in the Bacteroidetes and Firmicutes abundance between stages, while higher numbers of replicates were required for Actinobacteria (478 replicates) and Gammaproteobacteria (111 replicates).ConclusionsAlthough sample processing and extraction protocols may have had a significant influence, sampling is very important for studying the bee microbiome, and the importance of the number of individuals pooled in samples used for microbiome studies should not be underestimated.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/QJ1610248" target="_blank" >QJ1610248: Longevity of bees - its sustainability and role in beekeeping practice</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Microbiology
ISSN
1471-2180
e-ISSN
—
Volume of the periodical
19
Issue of the periodical within the volume
MAY 24
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
110
UT code for WoS article
000468899800001
EID of the result in the Scopus database
2-s2.0-85066396372