Comparison of enzymatic activities and proteomic profiles of Butyrivibrio fibrisolvens grown on different carbon sources

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F19%3A00506140" target="_blank" >RIV/67985904:_____/19:00506140 - isvavai.cz</a>

Alternative codes found

RIV/86652036:_____/19:00506140 RIV/67985823:_____/19:00506140 RIV/61388971:_____/19:00506140 RIV/00216208:11310/19:10395103

Result on the web

<a href="https://proteomesci.biomedcentral.com/articles/10.1186/s12953-019-0150-3" target="_blank" >https://proteomesci.biomedcentral.com/articles/10.1186/s12953-019-0150-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12953-019-0150-3" target="_blank" >10.1186/s12953-019-0150-3</a>

Result language

angličtina

Original language name

Comparison of enzymatic activities and proteomic profiles of Butyrivibrio fibrisolvens grown on different carbon sources

Original language description

Background: The rumen microbiota is one of the most complex consortia of anaerobes, involving archaea, bacteria, protozoa, fungi and phages. They are very effective at utilizing plant polysaccharides, especially cellulose and hemicelluloses. The most important hemicellulose decomposers are clustered with the genus Butyrivibrio. As the related species differ in their range of hydrolytic activities and substrate preferences, Butyrivibrio fibrisolvens was selected as one of the most effective isolates and thus suitable for proteomic studies on substrate comparisons in the extracellular fraction. The B. fibrisolvens genome is the biggest in the butyrivibria cluster and is focused on environmental information processing and carbohydrate metabolism. nMethods: The study of the effect of carbon source on B. fibrisolvens 3071 was based on cultures grown on four substrates: xylose, glucose, xylan, xylan with 25% glucose. The enzymatic activities were studied by spectrophotometric and zymogram methods. Proteomic study was based on genomics, 2D electrophoresis and nLC/MS (Bruker Daltonics) analysis. nResults: Extracellular beta-endoxylanase as well as xylan beta-xylosidase activities were induced with xylan. The presence of the xylan polymer induced hemicellulolytic enzymes and increased the protein fraction in the interval from 40 to 80kDa. 2D electrophoresis with nLC/MS analysis of extracellular B. fibrisolvens 3071 proteins found 14 diverse proteins with significantly different expression on the tested substrates. nConclusion: The comparison of four carbon sources resulted in the main significant changes in B. fibrisolvens proteome occurring outside the fibrolytic cluster of proteins. The affected proteins mainly belonged to the glycolysis and protein synthesis cluster.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Proteome Science

ISSN

1477-5956

e-ISSN

—

Volume of the periodical

17

Issue of the periodical within the volume

JUN 1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

2

UT code for WoS article

000469813700001

EID of the result in the Scopus database

2-s2.0-85066870472