Advantages of label free method in comparison with 2DE proteomic analysis of Butyrivibrio fibrisolvens 3071 grown on different carbon sources

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00563989" target="_blank" >RIV/67985904:_____/22:00563989 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/22:00563989 RIV/60460709:41210/22:92366

Result on the web

<a href="https://www.tandfonline.com/doi/full/10.1080/1828051X.2022.2129477" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/1828051X.2022.2129477</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/1828051X.2022.2129477" target="_blank" >10.1080/1828051X.2022.2129477</a>

Result language

angličtina

Original language name

Advantages of label free method in comparison with 2DE proteomic analysis of Butyrivibrio fibrisolvens 3071 grown on different carbon sources

Original language description

The aim of this study was the comparison of label-free method with 2DE to other analytical method of bacterium Butyrivibrio fibrisolvens extracellular protein samples. Label-free quantification (LF) method performed in this work was compared with previously obtained results of proteomic analysis using two-dimensional electrophoresis (2DE) and nano-liquid chromatography coupled with mass spectroscopy (nLC/MS). B. fibrisolvens as an important plant fibre degrader was cultivated on four different carbon sources (including xylan, xylose, glucose, and a mixture of xylan with glucose). The impact of growth substrate on protein profile was assessed by six pair-wise comparisons evaluating the significantly differently abundant extracellular proteins. Gel-free and gel-based methods resulted in substantially dissimilar results. The LC-MS/MS approach detected substrate-dependent differences in transport and binding membrane proteins (TBP) and nucleotidase, while the 2DE approach detected substrate effect on proteins included in protein synthesis and butyrate synthesis. On the other hand, both methods observed differentially regulated proteins involved in the glycolytic pathway, however, the only shared enzyme (protein detected both by 2DE and LC-MS/MS approach) was fructose-bisphosphate aldolase. The LC-MS/MS approach detected a high abundance of separated peptides. However, it cannot be easily considered as supreme to 2DE analysis. Both methods differ in sample preparation, their advantages and limitations. The study findings indicate these methods can complement each other and together they elucidate better the metabolic functions of B. fibrisolvens.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/EF15_003%2F0000460" target="_blank" >EF15_003/0000460: EXCELLENCE in Molecular Aspects of the early development of vertebrates</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Italian Journal of Animal Science

ISSN

1594-4077

e-ISSN

1828-051X

Volume of the periodical

21

Issue of the periodical within the volume

1

Country of publishing house

IT - ITALY

Number of pages

12

Pages from-to

1508-1519

UT code for WoS article

000868161000001

EID of the result in the Scopus database

2-s2.0-85148463551