Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F19%3A00511982" target="_blank" >RIV/67985904:_____/19:00511982 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10406965
Result on the web
<a href="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=41048410983" target="_blank" >https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=41048410983</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1159/000496171" target="_blank" >10.1159/000496171</a>
Alternative languages
Result language
angličtina
Original language name
Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly
Original language description
We developed new tools to build a high-quality chromosomal map of the Komodo dragon (Varanus komodoensis) available for cross-species phylogenomic analyses. First, we isolated chromosomes by flow sorting and determined the chromosome content of each flow karyotype peak by FISH. We then isolated additional Komodo dragon chromosomes by microdissection and amplified chromosome-specific DNA pools. The chromosome-specific DNA pools can be sequenced, assembled, and mapped by next-generation sequencing technology. The chromosome-specific paint probes can be used to investigate karyotype evolution through cross-species chromosome painting. Overall, the set of chromosome-specific DNA pools of V. komodoensis provides new tools for detailed phylogenomic analyses of Varanidae and squamates in general.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10613 - Zoology
Result continuities
Project
<a href="/en/project/GJ17-22141Y" target="_blank" >GJ17-22141Y: Evolution of sex chromosomes in toxicoferan reptiles</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytogenetic and Genome Research
ISSN
1424-8581
e-ISSN
—
Volume of the periodical
157
Issue of the periodical within the volume
1-2
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
123-131
UT code for WoS article
000466128800013
EID of the result in the Scopus database
2-s2.0-85060205083