CKS1 Germ Line Exclusion Is Essential for the Transition from Meiosis to Early Embryonic Development
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F19%3A00512107" target="_blank" >RIV/67985904:_____/19:00512107 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10403763
Result on the web
<a href="https://mcb.asm.org/content/39/13/e00590-18" target="_blank" >https://mcb.asm.org/content/39/13/e00590-18</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/MCB.00590-18" target="_blank" >10.1128/MCB.00590-18</a>
Alternative languages
Result language
angličtina
Original language name
CKS1 Germ Line Exclusion Is Essential for the Transition from Meiosis to Early Embryonic Development
Original language description
Cell division cycle (cdc) kinase subunit (CKS) proteins bind cyclin-dependent kinases (CDKs) and play important roles in cell division control and development, though their precise molecular functions are not fully understood. Mammals express two closely related paralogs called CKS1 and CKS2, but only CKS2 is expressed in the germ line, indicating that it is solely responsible for regulating CDK functions in meiosis. Using cks2(-/-) knockout mice, we show that CKS2 is a crucial regulator of maturation-promoting factor (MPF, CDK1-cyclin A/B) activity in meiosis. cks2(-/-) oocytes display reduced and delayed MPF activity during meiotic progression, leading to defects in germinal vesicle breakdown (GVBD), anaphase-promoting complex/cyclosome (APC/C) activation, and meiotic spindle assembly. cks2(-/-) germ cells express significantly reduced levels of the MPF components CDK1 and cyclins A1/B1. Additionally, injection of MPF plus CKS2, but not MPF alone, restored normal GVBD in cks2(-/- )oocytes, demonstrating that GVBD is driven by a CKS2-dependent function of MPF. Moreover, we generated cks2(cks1/cks1)( )knock-in mice and found that CKS1 can compensate for CKS2 in meiosis in vivo, but homozygous embryos arrested development at the 2- to 5-cell stage. Collectively, our results show that CKS2 is a crucial regulator of MPF functions in meiosis and that its paralog, CKS1, must be excluded from the germ line for proper embryonic development.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular and Cellular Biology
ISSN
0270-7306
e-ISSN
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Volume of the periodical
39
Issue of the periodical within the volume
13
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
UNSP e00590-18
UT code for WoS article
000471243000005
EID of the result in the Scopus database
2-s2.0-85068118528