The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F20%3A00525646" target="_blank" >RIV/67985904:_____/20:00525646 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/20:00525646

Result on the web

<a href="https://academic.oup.com/nar/article/48/6/3211/5709715" target="_blank" >https://academic.oup.com/nar/article/48/6/3211/5709715</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkz1239" target="_blank" >10.1093/nar/gkz1239</a>

Result language

angličtina

Original language name

The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution

Original language description

Tens of thousands of rapidly evolving long non-coding RNA (lncRNA) genes have been identified, but functions were assigned to relatively few of them. The lncRNA contribution to the mouse oocyte physiology remains unknown. We report the evolutionary history and functional analysis of Sirena1, the most expressed lncRNA and the 10th most abundant poly(A) transcript in mouse oocytes. Sirena1 appeared in the common ancestor of mouse and rat and became engaged in two different post-transcriptional regulations. First, antisense oriented Bob pseudogene insertion into Sirena1 exon 1 is a source of small RNAs targeting Sob mRNA via RNA interference. Second, Sirena1 evolved functional cytoplasmic polyadenylation elements, an unexpected feature borrowed from translation control of specific maternal mRNAs. Sirena1 knock-out does not affect fertility, but causes minor dysregulation of the maternal transcriptome. This includes increased levels of Elob and mitochondrial mRNAs. Mitochondria in Sirena1(-/-) oocytes disperse from the perinuclear compartment, but do not change in number or ultrastructure. Taken together, Sirenal contributes to RNA interference and mitochondrial aggregation in mouse oocytes. Sirena1 exemplifies how lncRNAs stochastically engage or even repurpose molecular mechanisms during evolution. Simultaneously, Sirenai1 expression levels and unique functional features contrast with the lack of functional importance assessed under laboratory conditions.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

—

Volume of the periodical

48

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

3211-3227

UT code for WoS article

000525957500035

EID of the result in the Scopus database

—