A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F20%3A00536914" target="_blank" >RIV/67985904:_____/20:00536914 - isvavai.cz</a>

Alternative codes found

RIV/68081715:_____/20:00536914

Result on the web

<a href="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=820955896" target="_blank" >https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=820955896</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21228738" target="_blank" >10.3390/ijms21228738</a>

Result language

angličtina

Original language name

A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response

Original language description

The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO3)(2) NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover the effects of their exposure on individual target organs and to reveal potential variability in the lead clearance. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs. In the lungs and liver, Pb(NO3)(2) NP inhalation induced serious structural changes and their damage was present even after a 5-week clearance period despite the lead having been almost completely eliminated from the tissues. The numbers of macrophages significantly decreased after 11-week Pb(NO3)(2) NP inhalation, conversely, abundance of alpha-smooth muscle actin (alpha-SMA)-positive cells, which are responsible for augmented collagen production, increased in both tissues. Moreover, the expression of nuclear factor kappa B (NF-kappa B) and selected cytokines, such as tumor necrosis factor alpha (TNF alpha), transforming growth factor beta 1 (TGF beta 1), interleukin 6(IL-6), IL-1 alpha and IL-1 beta , displayed a tissue-specific response to lead exposure. In summary, diminished inflammatory response in tissues after Pb(NO3)(2) NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by sustained pathological changes in target organs, even after long clearance period.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

<a href="/en/project/GA20-02203S" target="_blank" >GA20-02203S: Analysis of tissue response to metal nanoparticles inhalation and mechanisms of their clearance</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Volume of the periodical

21

Issue of the periodical within the volume

22

Country of publishing house

CH - SWITZERLAND

Number of pages

27

Pages from-to

8738

UT code for WoS article

000594717000001

EID of the result in the Scopus database

2-s2.0-85096315776