Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00560190" target="_blank" >RIV/67985904:_____/22:00560190 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/22:00560190 RIV/68081715:_____/22:00560190 RIV/68378050:_____/22:00560190 RIV/00216224:14310/22:00126406
Result on the web
<a href="https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-022-00494-7" target="_blank" >https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-022-00494-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12989-022-00494-7" target="_blank" >10.1186/s12989-022-00494-7</a>
Alternative languages
Result language
angličtina
Original language name
Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters
Original language description
Background: Inhalation of lead oxide nanoparticles (PbO NPs), which are emitted to the environment by high-temperature technological processes, heavily impairs target organs. These nanoparticles pass through the lung barrier and are distributed via the blood into secondary target organs, where they cause numerous pathological alterations. Here, we studied in detail, macrophages as specialized cells involved in the innate and adaptive immune response in selected target organs to unravel their potential involvement in reaction to subchronic PbO NP inhalation. In this context, we also tackled possible alterations in lipid uptake in the lungs and liver, which is usually associated with foam macrophage formation.nResults: The histopathological analysis of PbO NP exposed lung revealed serious chronic inflammation of lung tissues. The number of total and foam macrophages was significantly increased in lung, and they contained numerous cholesterol crystals. PbO NP inhalation induced changes in expression of phospholipases C (PLC) as enzymes linked to macrophage-mediated inflammation in lungs. In the liver, the subchronic inhalation of PbO NPs caused predominantly hyperemia, microsteatosis or remodeling of the liver parenchyma, and the number of liver macrophages also significantly was increased. The gene and protein expression of a cholesterol transporter CD36, which is associated with lipid metabolism, was altered in the liver. The amount of selected cholesteryl esters (CE 16:0, CE 18:1, CE 20:4, CE 22:6) in liver tissue was decreased after subchronic PbO NP inhalation, while total and free cholesterol in liver tissue was slightly increased. Gene and protein expression of phospholipase PLC beta 1 and receptor CD36 in human hepatocytes were affected also in in vitro experiments after acute PbO NP exposure. No microscopic or serious functional kidney alterations were detected after subchronic PbO NP exposure and CD68 positive cells were present in the physiological mode in its interstitial tissues.nConclusion: Our study revealed the association of increased cholesterol and lipid storage in targeted tissues with the alteration of scavenger receptors and phospholipases C after subchronic inhalation of PbO NPs and yet uncovered processes, which can contribute to steatosis in liver after metal nanoparticles exposure.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30108 - Toxicology
Result continuities
Project
<a href="/en/project/GA20-02203S" target="_blank" >GA20-02203S: Analysis of tissue response to metal nanoparticles inhalation and mechanisms of their clearance</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Particle and Fibre Toxicology
ISSN
1743-8977
e-ISSN
1743-8977
Volume of the periodical
19
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
28
Pages from-to
52
UT code for WoS article
000835708500001
EID of the result in the Scopus database
2-s2.0-85135358242