Characterization of porcine oocytes stained with Lissamine Green B and their developmental potential in vitro
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F20%3A00550729" target="_blank" >RIV/67985904:_____/20:00550729 - isvavai.cz</a>
Result on the web
<a href="https://www.scielo.br/j/ar/a/7ywdBxWN8pbPVk4FC8WgtrJ/?lang=en" target="_blank" >https://www.scielo.br/j/ar/a/7ywdBxWN8pbPVk4FC8WgtrJ/?lang=en</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1590/1984-3143-AR2020-0533" target="_blank" >10.1590/1984-3143-AR2020-0533</a>
Alternative languages
Result language
angličtina
Original language name
Characterization of porcine oocytes stained with Lissamine Green B and their developmental potential in vitro
Original language description
Traditional methods for the evaluation of oocyte quality are based on morphological classification of the follicle, cumulus-oocyte complex, polar body and meiotic spindle. This study is focused on the differences between the morphological assessment of oocyte quality, the assessment based on Lissamine Green B (LB) staining and the analysis of oocytes using a proteomic approach. We evaluated the effectiveness of electrochemical and chemical parthenogenetic activation under our laboratory conditions and evaluated the applicability of Lissamine Green B staining of cumulus-oocyte complexes (COCs) as a non-invasive method for predicting the maturational and developmental competence of porcine oocytes cultured in vitro. We determined that chemical parthenogenetic activation using ionomycin and 6-dimethylaminopurine was slightly more effective than electrochemical activation. After oocyte selection according to LB staining, we found significant differences (P<0.05) between the LB-group and LB+ group and the control group in their maturation, cleavage rate and rate of blastocysts. Proteomic analyses identified a selection of proteins that were differentially expressed in each group of analysed oocytes. Oocytes of the LB-group exhibited an increased variability of proteins involved in transcription regulation, proteosynthesis and the protein folding crucial for oocyte maturation and further embryonic development. These results found a better competence of LB-oocytes in maturation, cleavage and ability to reach the blastocyst stage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
<a href="/en/project/EF15_003%2F0000460" target="_blank" >EF15_003/0000460: EXCELLENCE in Molecular Aspects of the early development of vertebrates</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animal Reproduction
ISSN
1806-9614
e-ISSN
1984-3143
Volume of the periodical
17
Issue of the periodical within the volume
4
Country of publishing house
BR - BRAZIL
Number of pages
11
Pages from-to
e20200533
UT code for WoS article
000645124300010
EID of the result in the Scopus database
2-s2.0-85098937476