Implication of TRPC3 channel in gustatory perception of dietary lipids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00541169" target="_blank" >RIV/67985904:_____/21:00541169 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/21:00121009
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/apha.13554" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/apha.13554</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/apha.13554" target="_blank" >10.1111/apha.13554</a>
Alternative languages
Result language
angličtina
Original language name
Implication of TRPC3 channel in gustatory perception of dietary lipids
Original language description
Aim The pathogenesis of obesity has been associated with high intake of dietary fat, and some recent studies have explored the cellular mechanisms of oro-sensory detection of dietary fatty acids. We further assessed the role of transient receptor potential canonical (TRPC) channels in oro-sensory perception of dietary lipids. Methods We determined by RT-qPCR and western blotting the expression of TRPC3/6/7 channels in mouse fungiform taste bud cells (mTBC). Immunocytochemistry was used to explore whether TRPC3 channels were co-expressed with fatty acid receptors. We employed wild-type (WT) mTBC, and those transfected with small interfering RNAs (siRNAs) against TRPC3 or STIM1. Ca(2+)signalling was studied in TBC fromTRPC3(-/-)mice and their WT littermates. Results We demonstrate that mouse fungiform taste bud cells (mTBC) express TRPC3, but not TRPC6 or TRPC7 channels, and their inactivation by siRNA or experiments on TBC fromTRPC3(-/-)mice brought about a decrease in fatty acid-induced gustatory Ca(2+)signalling, coupled with taste bud CD36 lipid sensor. TRPC3 channel activation was found to be under the control of STIM1 in lingual mTBC. Behavioural studies showed that spontaneous preference for a dietary long-chain fatty acid was abolished inTRPC3(-/-)mice, and in mice wherein lingual TRPC3 expression was silenced by employing siRNA. Conclusion We report that lingual TRPC3 channels are critically involved in fat taste perception.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Physiologica
ISSN
1748-1708
e-ISSN
1748-1716
Volume of the periodical
231
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
e13554
UT code for WoS article
000568624500001
EID of the result in the Scopus database
2-s2.0-85090839841